spsJ [2018-02-13T15:25:16.000Z]

dTDP -glucose-4,6-dehydratase, spore coat polysaccharide synthesis

Locus: BSU37830

Isoelectric point: 6.29

Molecular weight: 35.38 kDa

Protein length: 315 aa

Gene length: 945 bp

Function: rhamnose biosynthesis, spore coat polysaccharide synthesis

Product: dTDP -glucose-4,6-dehydratase

Essential: no

E.C.: 4.2.1.46

Synonyms: ipa-72d

Genomic Context

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Categories containing this gene/protein

• Metabolism → Additional metabolic pathways → Miscellaneous metabolic pathways → Biosynthesis of rhamnose (for the exosporium)
• Lifestyles → Sporulation → Sporulation proteins → Sporulation proteins/ other

Gene

Coordinates: 3,884,292 → 3,885,239

The protein

Catalyzed reaction/ biological activity

• dTDP-glucose -→ dTDP-4-dehydro-6-deoxyglucose PubMed

Protein family

• sugar epimerase family (according to Swiss-Prot)

Paralogous protein(s)

• YfnG

Expression and Regulation

Operons

• Genes: spsA-spsB-spsC-spsD-spsE-spsF-spsG-spsI-spsJ-spsK-spsL
  Description: PubMed

  Sigma factors

  • SigE: sigma factor, PubMed, in SigE regulon
  • SigK: sigma factor, PubMed, in SigK regulon

  Regulatory mechanism

  • GerE: activation, PubMed, in GerE regulon

  Regulation

  • expressed during sporulation in the mother cell (SigE, SigK, GerE) PubMed

  Additional information

  • the mRNA is very stable (half-life > 15 min) PubMed

Biological materials

Mutant

• BKE37830 (ΔspsJ::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_TGTAATTAAATATGATTTTG, downstream forward: _UP4_AATGACCGATGAGAAGGTGA
• BKK37830 (ΔspsJ::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_TGTAATTAAATATGATTTTG, downstream forward: _UP4_AATGACCGATGAGAAGGTGA

References

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